Chaturvedi A K, Rao N G, McCoy F E
Forensic Sci Int. 1983 Nov-Dec;23(2-3):265-75. doi: 10.1016/0379-0738(83)90155-x.
Death of a 21-year-old man who was found in a shower stall in his residence is described in the study. At the scene, a 3/4 filled blue glass bottle labeled "Black Leaf 40" (an insecticide containing nicotine), a white plastic pitcher 1/3 full of thick white fluid, a beer mug 1/4 full of thick white fluid, and an empty carton of milk were found. In addition, a can of malathion and an empty bottle labeled caffeine also were found in the vicinity. Autopsy was performed, and the gross examinations of organs revealed no specific findings to account for the death. However, marked congestion in lung, liver, spleen and kidney were noted at microscopic level. Autolytic degenerative changes were also observed in stomach, small bowel and colon. Toxicological analyses of the autopsy samples (blood, urine, liver and gastric contents) revealed the presence of caffeine and nicotine in each sample. Malathion was found to be present only in gastric content. Caffeine and nicotine were analyzed by utilizing gas liquid chromatography-nitrogen phosphorus detector, while malathion was by gas liquid chromatography-flame photometric detector. Analyses of the fluids from the bottle, pitcher and mug disclosed the presence of nicotine in the concentrations of 17.8%, 3.7% and 5.7% (w/w), respectively. The fluids from the pitcher and mug also contained 2.7-2.9% malathion. Results conclude the death was associated with caffeine, nicotine and malathion.
该研究描述了一名21岁男子的死亡案例，他被发现死于其住所的淋浴间。在现场，发现了一个3/4满的蓝色玻璃瓶，标签为“黑叶40”（一种含尼古丁的杀虫剂），一个1/3满的白色塑料水壶，里面装着浓稠的白色液体，一个1/4满的啤酒杯，里面装着浓稠的白色液体，以及一个空牛奶盒。此外，在附近还发现了一罐马拉硫磷和一个标有咖啡因的空瓶子。进行了尸检，器官的大体检查未发现可解释死亡原因的具体发现。然而，在显微镜下观察到肺、肝、脾和肾有明显充血。在胃、小肠和结肠也观察到自溶退行性变化。对尸检样本（血液、尿液、肝脏和胃内容物）的毒理学分析显示，每个样本中都含有咖啡因和尼古丁。仅在胃内容物中发现了马拉硫磷。咖啡因和尼古丁采用气相色谱 - 氮磷检测器进行分析，而马拉硫磷采用气相色谱 - 火焰光度检测器进行分析。对瓶子、水壶和杯子中的液体分析表明，尼古丁浓度分别为17.8%、3.7%和5.7%（w/w）。水壶和杯子中的液体还含有2.7 - 2.9%的马拉硫磷。结果表明，死亡与咖啡因、尼古丁和马拉硫磷有关。
