Suppression of hepatic lipogenesis by pectin and galacturonic acid orally-fed at the separate timing from digestion-absorption of nutrients in rat.

This study was conducted to know the possibility that pectin-induced alterations in lipid metabolism of animals might be partly ascribed to galacturonic acid produced by the degradation of ingested pectin in the digestive tract. After a 4-week meal feeding twice a day, fasted rats were fed glucose and fructose and 3 h later orally administered 213 mg of pectin (from apple) or galacturonic acid per kg of body weight, or fed water alone. Significant changes in serum and liver lipids were observed 30 min and 1 h after the administration of pectin and galacturonic acid but not 5 h after the administration. Pectin and galacturonic acid showed contradictory effects on serum lipids, adipose tissue lipoprotein lipase activity and triacylglycerol (TG) production and removal rates. However, the elevation of total lipid and TG levels in liver with the sugar feeding was significantly inhibited by the administration of either pectin or galacturonic acid. These results support our hypothesis that galacturonic acid produced by the degradation of ingested pectin in the digestive tract may be partly responsible for the pectin-induced changes in lipid metabolism. This was discussed in relation to another possible regulation of lipid metabolism by short-chain fatty acids which are produced by the intestinal fermentation of pectin and galacturonic acid.