Ray J E, Day R O
J Pharm Sci. 1983 Dec;72(12):1403-5. doi: 10.1002/jps.2600721209.
A high-performance liquid chromatographic (HPLC) assay with fluorescence detection has been developed for the determination of diflunisal in plasma and urine. The plasma or urine, containing naproxen as the internal standard, was extracted with ether-hexane (1:1). The samples were analyzed on a microparticulate column, and the compounds were eluted using a mobile phase of 0.05 M phosphate buffer (pH 3) and methanol. Plasma samples were analyzed from two healthy male subjects who received a 250- and 750-mg oral dose of diflunisal 3 weeks apart. The data were analyzed according to a two-compartment open model. There was a disproportionate increase in the area under the plasma concentration-time curves (AUC 750 mg/AUC 250 mg was 3.84 for subject A and 4.22 for subject B) and a reduction in plasma clearance after the 750-mg dose of diflunisal. These data suggest that the kinetics of diflunisal may be dose dependent.
已开发出一种带荧光检测的高效液相色谱（HPLC）分析法，用于测定血浆和尿液中的二氟尼柳。含有萘普生作为内标的血浆或尿液用乙醚 - 己烷（1:1）萃取。样品在微粒柱上进行分析，化合物用0.05M磷酸盐缓冲液（pH 3）和甲醇的流动相洗脱。对两名健康男性受试者的血浆样本进行分析，他们相隔3周分别口服250毫克和750毫克二氟尼柳。数据根据二室开放模型进行分析。二氟尼柳750毫克剂量后，血浆浓度 - 时间曲线下面积出现不成比例的增加（受试者A的AUC 750毫克/AUC 250毫克为3.84，受试者B为4.22），并且血浆清除率降低。这些数据表明二氟尼柳的动力学可能与剂量有关。
