Yamamoto H, Tsutsui K, Shimada K, Yamanishi Y, Imai S
J Toxicol Sci. 1983 Nov;8(4):301-10. doi: 10.2131/jts.8.301.
Subacute toxicity of STO was carried out using Wistar rats. STO was administered intravenously at dose levels of 12.5, 50.0 and 200.0 mg/kg for 35 days. No influence on general symptom, body weight, food and water consumption and urinalysis were observed. In 200.0 mg/kg group, the hematological examination revealed anemia corresponding to the histological findings of the bone marrow. Biochemical analysis displayed the significant increase and/or decrease of enzyme values in 200.0 mg/kg group. Morphology showed the swelling, vacuolization and granuloma of the spleen, hepatic granuloma, the increase of reticulum cells and granuloma in the bone marrow. Embolism and thrombus were also found in the pulmonary and tail veins probably due to an artifact caused by the i.v. injection. The toxicity of the STO were observed only in 200.0 mg/kg group of both sexes and any remarkable change other than physical effects were found in other groups under the above dosage. Accordingly, the maximal no-effect level of the STO in Wistar rats was considered to be 50.0 mg/kg.
使用Wistar大鼠进行了STO的亚急性毒性试验。STO以12.5、50.0和200.0mg/kg的剂量水平静脉注射35天。未观察到对一般症状、体重、食物和水消耗以及尿液分析的影响。在200.0mg/kg组中，血液学检查显示与骨髓组织学结果相符的贫血。生化分析显示200.0mg/kg组中酶值显著升高和/或降低。形态学显示脾脏肿胀、空泡化和肉芽肿，肝脏肉芽肿，骨髓中网织细胞增多和肉芽肿。在肺静脉和尾静脉中也发现了栓塞和血栓，这可能是由于静脉注射引起的假象。仅在200.0mg/kg的雌雄组中观察到STO的毒性，在上述剂量下，其他组未发现除身体影响之外的任何显著变化。因此，Wistar大鼠中STO的最大无作用水平被认为是50.0mg/kg。
