Shibata Y, Arima T, Arima T, Yamamoto T
J Ultrastruct Res. 1983 Oct;85(1):70-81. doi: 10.1016/s0022-5320(83)90117-x.
Freeze-fracture deep-etch studies were done to examine regular patterns of surface membrane particles in the suckling rat ileal epithelium by using quick-freezing method. In addition to the presence on the luminal surfaces of well-developed endocytic complexes, latticed particles were consistently observed on almost the entire apical membrane between microvilli and frequently on the microvilli. These particles seemed to be partly integrated in the outer half layer of membranes. After rinsing with phosphate-buffered saline, patterns on the microvilli appeared to be parallel circle lines rather than latticed particles found in fresh preparations. Filipin treatment showed the presence of filipin-sterol complexes on most of the particle-covered membrane areas except small tubules and vesicles. Possibilities were suggested that these latticed particles were transferred to the apical surface along the outer half layer of membranes, and released or secreted into the intestinal lumen.
采用快速冷冻法进行冷冻断裂深度蚀刻研究，以检查乳鼠回肠上皮表面膜颗粒的规则模式。除了在管腔表面存在发育良好的内吞复合物外，在微绒毛之间的几乎整个顶端膜上以及微绒毛上经常一致地观察到晶格状颗粒。这些颗粒似乎部分整合在膜的外半层中。用磷酸盐缓冲盐水冲洗后，微绒毛上的模式似乎是平行的圆圈线，而不是新鲜制剂中发现的晶格状颗粒。制霉菌素处理表明，除了小管和小泡外，在大多数颗粒覆盖的膜区域存在制霉菌素 - 固醇复合物。有人提出，这些晶格状颗粒沿着膜的外半层转移到顶端表面，并释放或分泌到肠腔中。
