Lanthorn T H, Cotman C W
Neuropharmacology. 1983 Dec;22(12A):1343-8. doi: 10.1016/0028-3908(83)90222-8.
The relative potency of analogues of excitatory amino acids to produce depolarization when applied in the apical dendritic field of CA1 cells was studied in the hippocampal slice. The effect of these compounds was measured by recording focal potentials (FPs), the shift in the extracellular d.c. potential produced by the compounds applied. The ability of focal potentials to measure neuronal responses was evaluated. N-methyl-D-aspartate (NMDA)-type agonists were 10-20 times more potent, relative to L-glutamate, than reported from investigations in spinal cord. Quisqualate (QA), +/- alpha-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) and kainate (KA) exhibited potencies on CA1 cells similar to those reported for spinal neurons. These data indicate that elements in CA1 cells possess a receptor with an affinity for N-methyl-D-aspartate-type agonists. Some putative antagonists were found to induce negative focal potentials suggesting a direct, excitatory action in this area.
在海马切片中研究了兴奋性氨基酸类似物在应用于CA1细胞顶树突场时产生去极化的相对效力。通过记录局灶性电位（FPs）来测量这些化合物的作用，即所施加化合物引起的细胞外直流电位的变化。评估了局灶性电位测量神经元反应的能力。相对于L-谷氨酸，N-甲基-D-天冬氨酸（NMDA）型激动剂的效力比脊髓研究报告的高10 - 20倍。quisqualate（QA）、±α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）和海人藻酸（KA）对CA1细胞的效力与报道的脊髓神经元相似。这些数据表明，CA1细胞中的元件具有对N-甲基-D-天冬氨酸型激动剂有亲和力的受体。发现一些假定的拮抗剂会诱导负性局灶性电位，表明在该区域有直接的兴奋作用。
