Gwyn D G, Rutherford J G, Nicholson G P
Neurosci Lett. 1983 Dec 11;42(3):243-8. doi: 10.1016/0304-3940(83)90269-0.
Prior to the electron microscopic identification of spino-olivary terminals, the distribution of the direct spino-olivary projection was determined at the light microscopic level. Lesions were placed in the cervical or thoracic spinal cord in 7 rats. After a 7 day survival, animals were perfused, and transverse sections through the medulla were processed according to the Fink-Heimer technique. Terminal degeneration was found in the caudal third of the medial accessory olive, and in the lateral half of the dorsal accessory olive throughout its length. In order to determine the ultrastructural characteristics of direct spino-olivary terminals, lesions were placed in the cervical spinal cord of a further 4 rats. After a 2 or 3 day survival period, the animals were processed for electron microscopy. The portions of the inferior olivary nucleus in which degeneration had been noted in the Fink-Heimer studies were sampled and examined in the electron microscope. The majority of degenerating spino-olivary terminals underwent an electrondense change. Most degenerating terminals contained round, clear vesicles, but a few were found to contain predominantly flattened vesicles. Degenerating terminals contacted small dendritic profiles or spines, neither of which were associated with synaptic clusters.
在通过电子显微镜鉴定脊髓橄榄终末之前，先在光学显微镜水平确定直接脊髓橄榄投射的分布。对7只大鼠的颈段或胸段脊髓进行损伤。存活7天后，对动物进行灌注，并根据芬克 - 海默技术处理延髓的横切片。在内侧副橄榄核的尾侧三分之一以及背侧副橄榄核全长的外侧一半发现了终末变性。为了确定直接脊髓橄榄终末的超微结构特征，又对4只大鼠的颈段脊髓进行损伤。在2或3天的存活期后，对动物进行电子显微镜处理。对在芬克 - 海默研究中发现有变性的下橄榄核部分进行取样并在电子显微镜下检查。大多数变性的脊髓橄榄终末发生电子密度变化。大多数变性终末含有圆形、清亮的小泡，但发现少数主要含有扁平小泡。变性终末与小的树突状轮廓或棘突接触，两者均不与突触簇相关。
