Lauritzen E, Rubin I
Scand J Clin Lab Invest. 1983 Dec;43(8):715-21.
Angiotensin I (AI) protein complex with Mr 25,000 and an angiotensin I immunoreactive protein with Mr 60,000 were isolated from rat serum. The proteins were separated from AI by sodiumdodecylsulphate polyacrylamide gel electrophoresis under denaturing and reducing conditions using SDS and 2-mercaptoethanol (SDS-PAGE). The Mr 60,000 protein was isolated from non-incubated serum. Special attention was given to the Mr 25,000 AI-protein complex, called AI-25,000, which was generated in rat serum incubated with both synthetic and native AI for 1-5 h at 24 degrees C and neutral pH. The AI-25,000 was determined in two radioimmunoassays using two anti-AI antisera and 125I-AI. The experiments showed that AI reacts with a serum protein and forms a stable complex with a high molecular mass.
从大鼠血清中分离出分子量为25,000的血管紧张素I（AI）蛋白复合物以及分子量为60,000的血管紧张素I免疫反应性蛋白。在变性和还原条件下，使用十二烷基硫酸钠（SDS）和2-巯基乙醇，通过SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）将这些蛋白质与AI分离。分子量为60,000的蛋白质是从未孵育的血清中分离出来的。特别关注了分子量为25,000的AI-蛋白复合物，称为AI-25,000，它是在24℃和中性pH条件下，将合成的和天然的AI与大鼠血清孵育1-5小时后产生的。使用两种抗AI抗血清和125I-AI通过两种放射免疫测定法测定AI-25,000。实验表明，AI与一种血清蛋白反应并形成一种稳定的高分子量复合物。
