Bokhorov O, Lazareov I, Chotinski D
Vet Med Nauki. 1983;20(9):74-80.
The experiments were carried out with 4-week-old broiler birds given a ration that was balanced in terms of basic nutrients. Following decapitation inverted intestinal sacs were prepared from the proximal part of the jejunum the incubation of which lasted 40 min. To elucidate the effect of folic acid on the transport of 35S-methionine the acid was used in a conc. of 4 X 10(-6). Studied was also the effect of replacing the sodium ion (148 mEq/l) in the incubation solution with equivalent amount of the potassium ion, and the incorporation into the same solution of monoiodine-acetic acid (10(-3)M) as it affects the transmembrane transport of methionine. The flasks with the sacs of each group of birds contained 10microCi 35S-methionine each. The content of cyclic 3', 5'-adenosine-monophosphate (cAMP), the SH-groups, and the activity of alkaline phosphatase in the jejunal mucosa were determined. Results showed that folic acid at 4 X 10(-6)M stimulated dependably both the transport and the incorporation of 35S-methionine into the erythrocytes. These processes were inhibited when monoiodine-acetic acid at 10(-3)M was added to the incubation solution. Identic negative effect was produced also when K+ was used instead of Na+. Folic acid was said to raise the content of cAMP, SH-groups, and the activity of alkaline phosphatase in the jejunal mucosa.(ABSTRACT TRUNCATED AT 250 WORDS)
实验使用4周龄的肉鸡进行，给予其基础营养均衡的日粮。断头后，从空肠近端制备倒置肠囊，孵育持续40分钟。为阐明叶酸对35S-蛋氨酸转运的影响，使用浓度为4×10(-6)的叶酸。还研究了用等量钾离子替代孵育溶液中的钠离子(148 mEq/l)的效果，以及将一碘乙酸(10(-3)M)加入同一溶液中对蛋氨酸跨膜转运的影响。每组鸡的肠囊烧瓶中各含有10微居里的35S-蛋氨酸。测定空肠黏膜中环状3',5'-腺苷单磷酸(cAMP)、SH基团的含量以及碱性磷酸酶的活性。结果表明，4×10(-6)M的叶酸可靠地刺激了35S-蛋氨酸向红细胞的转运和掺入。当向孵育溶液中加入10(-3)M的一碘乙酸时，这些过程受到抑制。用K+代替Na+时也产生了相同的负面影响。据说叶酸会提高空肠黏膜中cAMP、SH基团的含量以及碱性磷酸酶的活性。(摘要截短至250字)
