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牛晶状体上皮细胞在体外的分化状态。细胞形状变化与晶状体蛋白合成之间的关系。

State of differentiation of bovine epithelial lens cells in vitro. Relationship between the variation of the cell shape and the synthesis of crystallins.

作者信息

Simonneau L, Herve B, Jacquemin E, Courtois Y

出版信息

Cell Differ. 1983 Nov;13(3):185-90. doi: 10.1016/0045-6039(83)90088-x.

DOI:10.1016/0045-6039(83)90088-x
PMID:6667494
Abstract

Correlations between cell morphology and the expression of specific proteins (crystallins) have been investigated. Two different culture conditions have been chosen which keep bovine epithelial lens cells (BEL cells) in a monolayer of homogeneous epithelioid cells: (1) bovine retinal extract (EDGF) supplemented medium; (2) extracellular matrix (ECM) provided by corneal endothelial cells in standard medium has been compared to previous results obtained with BEL cells cultivated on plastic (Simonneau et al., 1983). Variations of the cell shape had no effect upon crystallin synthesis.

摘要

已经研究了细胞形态与特定蛋白质(晶状体蛋白)表达之间的相关性。选择了两种不同的培养条件,可使牛晶状体上皮细胞(BEL细胞)保持在均匀上皮样细胞的单层中:(1)添加牛视网膜提取物(EDGF)的培养基;(2)将标准培养基中角膜内皮细胞提供的细胞外基质(ECM)与之前在塑料上培养的BEL细胞所获得的结果进行比较(Simonneau等人,1983年)。细胞形状的变化对晶状体蛋白合成没有影响。

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State of differentiation of bovine epithelial lens cells in vitro. Relationship between the variation of the cell shape and the synthesis of crystallins.牛晶状体上皮细胞在体外的分化状态。细胞形状变化与晶状体蛋白合成之间的关系。
Cell Differ. 1983 Nov;13(3):185-90. doi: 10.1016/0045-6039(83)90088-x.
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cAMP-dependent phosphorylation of bovine lens alpha-crystallin.牛晶状体α-晶状体蛋白的环磷酸腺苷(cAMP)依赖性磷酸化
Proc Natl Acad Sci U S A. 1985 Jul;82(14):4712-6. doi: 10.1073/pnas.82.14.4712.