Rajan R, Johnstone B M
Hear Res. 1983 Dec;12(3):405-17. doi: 10.1016/0378-5955(83)90009-6.
We report a technique for activating the efferent nerve fibres to the cochlea by electrical stimulation at the round window. Such electrical stimulation caused a reduction in the amplitude of the gross nerve response (N1) to a click presented after the electrical stimulus but did not alter the latency of the response. The reduction increased with increasing current strength above 200 microA and increasing rate of electrical pulses above 50 Hz. The effect was also dependent on the duration of the shock train and the pulse width. The reduction in N1 was most pronounced at low click intensities. Recovery of the N1 was almost complete about 80 ms after the end of the electrical stimulus. The effect of electrical stimulation in reducing the N1 amplitude could almost always be blocked by intraperitoneal injections of strychnine. Recovery from the strychnine block was observed when animals were maintained for periods of more than 60 min after the administration of strychnine. The ease of this technique allows it to be used to examine the effects of efferent stimulation on various aspects of cochlear function in the guinea pig.
我们报告了一种通过在圆窗进行电刺激来激活耳蜗传出神经纤维的技术。这种电刺激导致在电刺激后呈现的短声诱发的总神经反应（N1）幅度降低，但未改变反应的潜伏期。当电流强度超过200微安且电脉冲频率超过50赫兹时，这种降低幅度随电流强度增加和电脉冲频率增加而增大。该效应还取决于电刺激串的持续时间和脉冲宽度。N1的降低在低声强时最为明显。电刺激结束后约80毫秒，N1几乎完全恢复。腹腔注射士的宁几乎总能阻断电刺激降低N1幅度的效应。在给予士的宁后，当动物维持超过60分钟时，可观察到从士的宁阻断中恢复。这种技术操作简便，可用于研究传出刺激对豚鼠耳蜗功能各个方面的影响。
