一种分离第四补体成分的简化技术。

A simplified technique for the isolation of the fourth complement component.

作者信息

Steinbuch M, Kichenin V, Péjaudier L

出版信息

Prep Biochem. 1983;13(5):507-18. doi: 10.1080/10826068308070606.

Abstract

Prothrombin complex (P.C.) preparations obtained by batch adsorption onto DEAE-Sephadex are highly enriched in C4. Based on this observation a technique has been elaborated where the P.C. analogue is further submitted to precipitation by ethanol and batch adsorption of impurities on DEAE-Cellulose. Unaltered C4 is obtained in a 2 days process with a yield corresponding to 40% of the starting material (cryoconcentrate supernatant).

摘要

通过分批吸附到二乙氨基乙基葡聚糖（DEAE - Sephadex）上获得的凝血酶原复合物（P.C.）制剂富含C4。基于这一观察结果，已精心设计了一种技术，其中将P.C.类似物进一步用乙醇沉淀，并将杂质分批吸附到二乙氨基乙基纤维素（DEAE - Cellulose）上。在一个为期2天的过程中可获得未改变的C4，产率相当于起始材料（冷冻浓缩上清液）的40%。