Loftus J C, Albrecht R M
Scan Electron Microsc. 1983(Pt 4):1995-9.
Fibrinogen labelled colloidal gold was used in a direct labelling procedure with surface activated human platelets. Utilizing this technique, the platelet membrane receptor for fibrinogen was visualized by both scanning and high voltage electron microscopy. Changes in the degree of fibrinogen binding and the whole cell distribution of the fibrinogen receptor are associated with the progression of the morphological transformation induced following platelet activation. While unstimulated platelets do not bind fibrinogen, the amount of fibrinogen bound per platelet increases rapidly during the early stages of shape change characteristic of surface activation. Redistribution of fibrinogen receptors to the central areas of platelets occurs following saturation of receptor sites. The ease of preparation of the label and its easy detection by electron microscopy make it useful for correlative HVEM and SEM studies of the relationship between receptor redistribution and cytoplasmic ultrastructural reorganization.
用纤维蛋白原标记的胶体金对表面活化的人血小板进行直接标记。利用该技术，通过扫描电子显微镜和高压电子显微镜观察到了纤维蛋白原的血小板膜受体。纤维蛋白原结合程度的变化以及纤维蛋白原受体在整个细胞中的分布与血小板活化后诱导的形态转变进程相关。未受刺激的血小板不结合纤维蛋白原，在表面活化特征性形状改变的早期阶段，每个血小板结合的纤维蛋白原量迅速增加。受体位点饱和后，纤维蛋白原受体重新分布到血小板的中心区域。该标记物易于制备且通过电子显微镜易于检测，这使其可用于对受体重新分布与细胞质超微结构重组之间关系进行相关的高压电子显微镜和扫描电子显微镜研究。
