使用激光荧光检测系统灵敏高效液相色谱法测定大鼠脑中的儿茶酚胺。

Sensitive high-performance liquid chromatographic determination of catecholamines in rat brain using a laser fluorimetric detection system.

作者信息

Todoriki H, Hayashi T, Naruse H, Hirakawa A Y

出版信息

J Chromatogr. 1983 Aug 12;276(1):45-54. doi: 10.1016/s0378-4347(00)85064-1.

PMID:6672024

Abstract

A sensitive high-performance liquid chromatographic method for the determination of catecholamines in rat brains has been developed using a fluorescence detector equipped with a continuous wave laser as an excitation light source. A new pre-purification and derivatization method was established and confirmed to be useful for the determination of catecholamines in biological samples. This pre-treatment method was simple, reproducible and specific. About 1 mg of the rat brain tissue was enough to determine catecholamines levels. The levels of dopamine and norepinephrine in rat brain were 0.40 and 0.87 ng, respectively, which agree with the findings of other workers.

摘要

已开发出一种灵敏的高效液相色谱法，用于测定大鼠脑中的儿茶酚胺，该方法使用配备连续波激光作为激发光源的荧光检测器。建立了一种新的预纯化和衍生化方法，并证实该方法可用于生物样品中儿茶酚胺的测定。这种预处理方法简单、可重复且具有特异性。约1mg大鼠脑组织足以测定儿茶酚胺水平。大鼠脑中多巴胺和去甲肾上腺素的水平分别为0.40和0.87ng，这与其他研究人员的发现一致。