Frank H, Vujtovic-Ockenga N, Rettenmeier A
J Chromatogr. 1983 Nov 25;279:507-14. doi: 10.1016/s0021-9673(01)93651-9.
Amino acid analysis by enantiomer labelling and capillary gas chromatography on Chirasil-Val is superior to conventional gas chromatography and ion-exchange chromatography with respect to sensitivity, accuracy and speed. Employment of an alkali flame-ionization detector allows the selective detection of amino acids and suppression of background peaks; in addition, the detectability of amino acids is enhanced. Most nitrogen-selective detectors require meticulous adjustment of the operating conditions, but in combination with enantiomer labelling this is less critical. Maximum sensitivity and selectivity of the alkali-bead flame-ionization detector is achieved with a minimal flow of hydrogen. When using it as the carrier gas in capillary gas chromatography, flow control instead of the common pressure regulation is recommended to avoid a continuous fall of the baseline during temperature programming. Accurate flow control is achieved with a micro-aperture. The benefit of nitrogen-selective detection is especially apparent for histidine and arginine.
通过对映体标记和在Chirasil-Val上进行毛细管气相色谱法进行氨基酸分析，在灵敏度、准确性和速度方面优于传统气相色谱法和离子交换色谱法。使用碱火焰离子化检测器可实现对氨基酸的选择性检测并抑制背景峰；此外，还增强了氨基酸的可检测性。大多数氮选择性检测器需要精心调整操作条件，但与对映体标记相结合时，这一点就不那么关键了。使用最小的氢气流可实现碱珠火焰离子化检测器的最大灵敏度和选择性。在毛细管气相色谱法中用作载气时，建议采用流量控制而非常见的压力调节，以避免程序升温期间基线持续下降。通过微孔实现精确的流量控制。氮选择性检测的优势在组氨酸和精氨酸的检测中尤为明显。
