Harel L, Blat C, Chatelain G
Biol Cell. 1983;48(1):11-6. doi: 10.1111/j.1768-322x.1984.tb00197.x.
Different data have suggested that the density-dependent inhibition of growth in normal 3T3 cells is due to the release of inhibitory factor(s) into the medium. The present results provide more evidence supporting this hypothesis. From conditioned medium of dense cultures of 3T3 cells, we fractionated a thermolabile inhibitory diffusible factor (I.D.F.) with a molecular weight of about 40.000 This factor decreased the [11C]-inosine incorporation into purine nucleotides and into nucleic acids of stimulated 3T3 cells. DNA synthesis, determined by [11C]-thymidine incorporation into nucleic acids, and protein synthesis (determined by the protein content of the cultures maintained in the presence or absence of I.D.F.), were also decreased. The possibility that I.D.F. is involved in density-dependent inhibition of growth is discussed.
不同的数据表明，正常3T3细胞中生长的密度依赖性抑制是由于抑制因子释放到培养基中所致。目前的结果提供了更多支持这一假设的证据。从3T3细胞密集培养的条件培养基中，我们分离出一种热不稳定的抑制性可扩散因子（I.D.F.），其分子量约为40000。该因子减少了[11C]-肌苷掺入受刺激的3T3细胞的嘌呤核苷酸和核酸中。通过[11C]-胸苷掺入核酸来测定的DNA合成以及蛋白质合成（通过在有或没有I.D.F.存在的情况下培养物的蛋白质含量来测定）也减少了。文中讨论了I.D.F.参与生长的密度依赖性抑制的可能性。
