Vazquez-Nin G H, Ortega-Rangel J A, Echeverria O M, Parra M R, Jimenez-Garcia L F
Biol Cell. 1983;48(1):17-24. doi: 10.1111/j.1768-322x.1984.tb00198.x.
Morphological and quantitative changes of ribonucleoproteic (RNP) structures and chromatin are studied in the nuclei of undifferentiated cells, neuroblasts and neurons in several degrees of maturation, in order to relate them to the drastic modifications in transcription and/or RNA processing taking place during cell differentiation. Undifferentiated (matrix) cells of 2-day embryos differ from that of 4-day embryos in their nucleolar volume and in the amount of compact chromatin. These differences are interpreted as the earliest signs of neuroblast differentiation. All over the process of matrix cell-bipolar-multipolar neuroblast differentiation there is a large spreading of compact chromatin well before any important change in RNP structures or in nuclear volume. The most remarkable increase in nuclear volume and in the amount of RNP particles occurs during the differentiation of multipolar neuroblasts to immature neurons, which is characterized by large synaptogenic activity. The interpretation of these changes is discussed in connection, on one hand with the metabolic effects of synapses, and on the other hand with the variations of gene expression taking place in cell differentiation and under other natural and experimental situations.
研究了未分化细胞、神经母细胞以及不同成熟度神经元细胞核中核糖核蛋白（RNP）结构和染色质的形态学及定量变化，以便将它们与细胞分化过程中发生的转录和/或RNA加工的剧烈变化联系起来。2日龄胚胎的未分化（基质）细胞在核仁体积和致密染色质数量上与4日龄胚胎的不同。这些差异被解释为神经母细胞分化的最早迹象。在基质细胞-双极-多极神经母细胞分化的整个过程中，在RNP结构或核体积发生任何重要变化之前，致密染色质就有大量扩散。核体积和RNP颗粒数量最显著的增加发生在多极神经母细胞向未成熟神经元分化的过程中，其特征是具有大量的突触形成活性。一方面结合突触的代谢效应，另一方面结合细胞分化以及其他自然和实验情况下发生的基因表达变化，对这些变化进行了讨论。
