Contraception. 1983 Dec;28(6):527-41.
A cross-sectional study was undertaken to assess the long-term immunological sequelae of vasectomy with respect to humoral response as well as the cell-mediated immune response (CMI) to spermatozoa. In the humoral response, circulating immune-complexes (CICs) were also estimated and attempts were made to investigate whether the development of antibodies to spermatozoa had any association with the HLA system. Four different techniques, viz. tray agglutination test (TAT), spermatozoa immobilization test (SIT), cellular-radioimmunoassay (CRIA) and the enzyme-linked immunosorbent assay (ELISA) were used to estimate antibodies to spermatozoa. Serum samples were considered positive when at least two of the above mentioned techniques gave a positive response to spermatozoal antigens. The results indicated that 37.5% of the vasectomized men developed antibodies to spermatozoa. CICs were present in 12% of the vasectomized subjects and 4% of the controls. 59.3% of the vasectomized who showed the presence of CICs, were negative to the presence of antibodies to spermatozoa. The HLA antigen frequency in the vasectomized subjects did not differ in individuals with and without spermatozoal antibodies. The CMI response to spermatozoa was negative in vasectomized subjects.
进行了一项横断面研究，以评估输精管切除术在体液免疫反应以及对精子的细胞介导免疫反应（CMI）方面的长期免疫后遗症。在体液免疫反应中，还对循环免疫复合物（CIC）进行了评估，并试图研究精子抗体的产生是否与HLA系统有关。采用了四种不同技术，即玻片凝集试验（TAT）、精子制动试验（SIT）、细胞放射免疫测定（CRIA）和酶联免疫吸附测定（ELISA）来评估精子抗体。当上述技术中至少有两种对精子抗原呈阳性反应时，血清样本被视为阳性。结果表明，37.5%的输精管切除男性产生了精子抗体。12%的输精管切除受试者和4%的对照组存在CIC。在显示存在CIC的输精管切除者中，59.3%的人精子抗体呈阴性。输精管切除受试者中HLA抗原频率在有精子抗体和无精子抗体的个体之间没有差异。输精管切除受试者对精子的CMI反应为阴性。
