α-氯醇对家兔精子体外氧化果糖的影响。

The effect of alpha-chlorohydrin on the oxidation of fructose by rabbit spermatozoa in vitro.

作者信息

Ford S A, Jones A R

出版信息

Contraception. 1983 Dec;28(6):565-73. doi: 10.1016/0010-7824(83)90107-5.

Abstract

(R,S)-alpha-Chlorohydrin inhibits the oxidative metabolism of fructose in mature rabbit spermatozoa in vitro. This effect is not noticeable at concentrations of the compound up to 10 mM, is evident at 50 mM but at 100 mM is apparently accompanied by cell damage. At a concentration of 50 mM, (R,S)-alpha-chlorohydrin causes the specific inhibition of the enzyme glyceraldehyde 3-phosphate dehydrogenase and is metabolised by the spermatozoa to 3-chlorolactaldehyde of unknown configuration. Exogenous (R,S)-3-chlorolactaldehyde (5 mM and 10 mM) appears to inhibit glyceraldehyde 3-phosphate dehydrogenase in rabbit spermatozoa as well as affecting other metabolic pathways. The ineffectiveness of (R,S)-alpha-chlorohydrin as an anti-fertility agent in male rabbits may be due to the inability of the spermatozoa to produce a sufficient amount of the inhibitory metabolite (S)-3-chlorolactaldehyde.

摘要

（R，S）-α-氯甘油在体外抑制成熟兔精子中果糖的氧化代谢。在该化合物浓度高达10 mM时，这种作用不明显，在50 mM时明显，但在100 mM时显然伴随着细胞损伤。在50 mM浓度下，（R，S）-α-氯甘油会特异性抑制3-磷酸甘油醛脱氢酶，并被精子代谢为构型未知的3-氯乙醛。外源性（R，S）-3-氯乙醛（5 mM和10 mM）似乎也会抑制兔精子中的3-磷酸甘油醛脱氢酶，并影响其他代谢途径。（R，S）-α-氯甘油作为雄性兔抗生育剂无效，可能是由于精子无法产生足够量的抑制性代谢物（S）-3-氯乙醛。