Abe T, Forbes J T, Colley D G
J Parasitol. 1983 Dec;69(6):1001-5.
Natural killer cell (NK) activity of spleen cells from CBA/J and C57BL/6 mice infected with Schistosoma mansoni was assayed using 51Cr-labeled YAC-1 cells as target cells. No significant difference in NK activity was detected between infected and age-matched control spleen cells from 1 to 4 wk after infection. The NK-mediated cytotoxic abilities of both infected and age-matched control mice fell in parallel, as expected, on an age-related basis. However, on a cell-to-cell basis the splenic NK activity of mice infected from 8 to 18 wk was significantly less than that of age-matched control mice. Meanwhile, because of the splenomegaly associated with patent infection, the total number of nucleated splenocytes in chronically infected mice increased to fourfold that of age-matched control mice. Therefore, the total available NK activity per spleen was increased by chronic infection. The NK activities of spleen cells from age-matched control mice and those infected for 18 wk could be augmented by in vivo administration of polyinosinic-polycytidylic acid, indicating the continued responsiveness of NK cells from chronically infected mice to activation. Spleen cell NK activity in age-matched control and infected C57BL/6 mice exhibited a pattern similar to that of CBA/J mice.
以51Cr标记的YAC-1细胞作为靶细胞，检测感染曼氏血吸虫的CBA/J和C57BL/6小鼠脾细胞的自然杀伤细胞（NK）活性。感染后1至4周，感染组和年龄匹配的对照脾细胞之间未检测到NK活性的显著差异。正如预期的那样，在与年龄相关的基础上，感染组和年龄匹配的对照小鼠的NK介导的细胞毒性能力平行下降。然而，在细胞对细胞的基础上，8至18周感染的小鼠的脾NK活性明显低于年龄匹配的对照小鼠。同时，由于与显性感染相关的脾肿大，慢性感染小鼠中有核脾细胞的总数增加到年龄匹配对照小鼠的四倍。因此，慢性感染使每个脾脏的总可用NK活性增加。年龄匹配的对照小鼠和感染18周的小鼠的脾细胞NK活性可通过体内给予聚肌苷酸-聚胞苷酸而增强，这表明慢性感染小鼠的NK细胞对激活持续有反应。年龄匹配的对照和感染的C57BL/6小鼠的脾细胞NK活性表现出与CBA/J小鼠相似的模式。
