未提取牛血浆中孕酮的快速放射免疫测定法。
Rapid radioimmunoassay of progesterone in unextracted bovine plasma.
作者信息
Sugden E A
出版信息
Can J Comp Med. 1978 Apr;42(2):229-34.
A rapid radioimmunoassay for determining progesterone levels in unextracted bovine plasma has been developed. Two antisera have been tested, anti-deoxycorticosterone-21-hemisuccinate human serum albumin and antiprogesterone-11-hemisuccinate bovine serum albumin. The usefulness of an antiserum for rapid assays has been based on its agreement with assays of organic extracts or chromatographically purified extracts of selected bovine samples. To avoid large blank effects and non-specific interference, a plasma volume of 20 microliter that provided adequate sensitivity was not exceeded. All correlations between rapid assays, assays using organic extraction or assays after chromatographic purification were greater than 0.9 and showed regression slopes approximating unity. It was concluded that individual lots of antisera should be evaluated thoroughly before use in a rapid assay.
已开发出一种用于测定未提取牛血浆中孕酮水平的快速放射免疫分析法。已测试了两种抗血清，即抗 -21-半琥珀酸脱氧皮质酮人血清白蛋白和抗 -11-半琥珀酸孕酮牛血清白蛋白。抗血清用于快速分析的有效性基于其与选定牛样品的有机提取物或色谱纯化提取物分析结果的一致性。为避免大的空白效应和非特异性干扰，未超过提供足够灵敏度的20微升血浆体积。快速分析、使用有机萃取的分析或色谱纯化后的分析之间的所有相关性均大于0.9，且回归斜率接近1。得出的结论是，在用于快速分析之前，应彻底评估各批次的抗血清。
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