Mahadevan M M, Trounson A O
Clin Reprod Fertil. 1983 Sep;2(3):217-27.
The effects of dilution and cryopreservation on biochemical, fine structural and conventional semen parameters were studied and correlated with an index of fertility. Dilution of semen had major effects on the parameters studied. Freezing and thawing further reduced motility and the proportion of viable spermatozoa, and increased the proportion of spermatozoa with damaged membranes. The fertility index of donors was positively correlated with sperm concentration, seminal plasma protein concentration, damage to sperm head membranes and the proportion of spermatozoa with intact acrosomes prior to dilution or after freezing and thawing. Seminal plasma [Na+], [Ca2+], Ca2+/Mg2+-ratios and the change in [K+] were negatively correlated with fertility index. Multiple regression analysis revealed that [Na+] in seminal plasma, and sperm concentration in semen prior to dilution and freezing, contribute to 68% of the variation in fertility of cryopreserved semen.
研究了精液稀释和冷冻保存对生化、精细结构及常规精液参数的影响，并将其与生育指数相关联。精液稀释对所研究的参数有重大影响。冷冻和解冻进一步降低了精子活力和活精子比例，并增加了膜受损精子的比例。供体的生育指数与稀释前或冷冻解冻后的精子浓度、精浆蛋白浓度、精子头部膜损伤以及顶体完整的精子比例呈正相关。精浆中的[Na+]、[Ca2+]、Ca2+/Mg2+比率以及[K+]的变化与生育指数呈负相关。多元回归分析显示，精浆中的[Na+]以及稀释和冷冻前精液中的精子浓度，占冷冻保存精液生育能力变异的68%。
