[Separation and analysis of ribonucleotide mixtures by anion exchange high performance liquid chromatography].

Ribonucleotides in the artificial mixture or from natural extracts were separated by HPLC on a column with strong anion-exchanger in gradient of pH and concentration of phosphate-chloride buffer system. Peaks were detected by UV-absorbance at 254 nm and detector sensitivity of 0,02 optical units for a full scale, whereas their identification was based on comparison of retention times with those of pure standards. An electronic integrator calibrated for each nucleotide was used for quantitative analysis. The conditions for separating 13 ribonucleotides (including IMP) during 40-minute HPLC-analysis were specified. Some problems pertinent to nucleotide extractions from biological samples are discussed.