Johnson D C, Griswold T
Steroids. 1983 Nov;42(5):565-74. doi: 10.1016/0039-128x(83)90120-4.
Immature hypophysectomized rats were injected with PMS; some groups received hCG 48h later. The C17,20-lyase activity in the granulosa cells removed from the large preovulatory follicles was estimated by the amount of labelled acetic acid produced from 21 (14C) progesterone or 17-hydroxyprogesterone. 17 alpha-hydroxylase and aromatase activity were measured by the tritium exchange method. Although the granulosa cells contained lyase, it was considerably less than their hydroxylase activity. The remaining tissue, consisting of small follicles and hypertrophied thecal and interstitial tissue, had a great deal more lyase and hydroxylase activity than did the granulosa cells. The results are consistent with the view that granulosa cells can produce estrogen from progesterone and do not require androgen precursors from the theca and/or interstitium.
对未成熟的垂体切除大鼠注射孕马血清促性腺激素（PMS）；部分组在48小时后注射人绒毛膜促性腺激素（hCG）。通过从21（14C）孕酮或17-羟孕酮产生的标记乙酸量来估计从大的排卵前卵泡中取出的颗粒细胞中的C17,20-裂解酶活性。通过氚交换法测量17α-羟化酶和芳香化酶活性。虽然颗粒细胞含有裂解酶，但其含量远低于其羟化酶活性。由小卵泡以及肥大的卵泡膜和间质组织组成的剩余组织，其裂解酶和羟化酶活性比颗粒细胞高得多。这些结果与以下观点一致，即颗粒细胞可以从孕酮产生雌激素，并且不需要来自卵泡膜和/或间质的雄激素前体。
