一种比色法测定血清中锌的新微量方法。

A new micromethod for colorimetric determination of zinc in serum.

作者信息

Ujiie M, Mikami I, Gemba T, Nomura A, Tokudome M, Fujii K

出版信息

Clin Chim Acta. 1978 Jul 1;87(1):71-6. doi: 10.1016/0009-8981(78)90059-1.

Abstract

A simple micromethod with a colorimetric reagent of high sensitivity was developed for the determination of zinc in serum. For the color reagent 2-(2-pyridylazo)-5-dimethylaminophenol (PADAP) was used. Coefficients of variation of the within-day precision were 6.65 and 2.24% for zinc standard at concentrations of 0.1 and 0.5 microgram/ml, respectively. The mean recovery by this method was 101.2% (S.D.: 3.56%). Zinc concentrations measured by the above method showed good agreement with those by atomic absorption spectrophotometry, with a correlation coefficient of 0.934. Serum zinc values of 50 healthy adults (38 females, 12 males) ranged from 63 to 116 microgram/100 ml (mean: 89.68 microgram/100 ml, Sd.: 13.28 microgram/100 ml).

摘要

开发了一种使用高灵敏度比色试剂的简单微量法用于测定血清中的锌。使用的比色试剂为2-(2-吡啶偶氮)-5-二甲基氨基酚（PADAP）。浓度为0.1和0.5微克/毫升的锌标准品日内精密度变异系数分别为6.65%和2.24%。该方法的平均回收率为101.2%（标准差：3.56%）。通过上述方法测得的锌浓度与原子吸收分光光度法测得的结果具有良好的一致性，相关系数为0.934。50名健康成年人（38名女性，12名男性）的血清锌值范围为63至116微克/100毫升（平均值：89.68微克/100毫升，标准差：13.28微克/100毫升）。