Couchman J R, Höök M, Rees D A, Timpl R
J Cell Biol. 1983 Jan;96(1):177-83. doi: 10.1083/jcb.96.1.177.
Human embryonic skin fibroblasts have been shown to attach and spread on laminin substrates in the absence of protein synthesis and presence of fibronectin-depleted serum and anti-fibronectin antibodies. Rates of attachment and the type of spreading are virtually identical on fibronectin and laminin-coated substrates with the development of microfilament bundles and focal adhesions. Antibodies to laminin, but not fibronectin, will prevent or reverse fibroblast adhesion to laminin, whereas antibodies to fibronectin but not laminin will give similar results on fibronectin-coated substrates. These and other results indicate that fibroblasts possess distinct receptors for laminin and fibronectin which on contact with suitable substrates promote adhesion through interaction with common intermediates. This type of adhesion is compatible with subsequent growth and extracellular matrix production.
已表明，在缺乏蛋白质合成、存在纤连蛋白缺失血清和抗纤连蛋白抗体的情况下，人胚胎皮肤成纤维细胞能够附着并铺展在层粘连蛋白底物上。在纤连蛋白和层粘连蛋白包被的底物上，随着微丝束和粘着斑的形成，附着速率和铺展类型实际上是相同的。抗层粘连蛋白抗体而非抗纤连蛋白抗体可阻止或逆转成纤维细胞与层粘连蛋白的粘附，而抗纤连蛋白抗体而非抗层粘连蛋白抗体在纤连蛋白包被的底物上会产生类似结果。这些及其他结果表明，成纤维细胞拥有层粘连蛋白和纤连蛋白的不同受体，这些受体在与合适底物接触时，通过与共同中间体相互作用促进粘附。这种粘附类型与随后的生长和细胞外基质产生是相容的。
