一种用于增殖淋巴细胞放射自显影研究的新方法。
A new approach to the autoradiographic study of proliferating lymphocytes.
作者信息
Zweiman B, Lisak R P
出版信息
Clin Exp Immunol. 1978 May;32(2):361-5.
Abstract
An adaptation of a cell filtration method for the autoradiographic study of cultured lymphocytes has been developed. The percentages of labelled cells are very similar in the filtered cell population to those obtained from replicate cultures processed by centrifugation. The filter method permitted a higher recovery of cells from small numbers in culture with better cytological detail than seen when cells were washed by repeated centrifugation, then suspended and smeared.
摘要
已开发出一种用于培养淋巴细胞放射自显影研究的细胞过滤方法的改良方法。过滤后的细胞群体中标记细胞的百分比与通过离心处理的重复培养物中获得的百分比非常相似。与通过反复离心洗涤细胞，然后悬浮并涂片相比，过滤方法能够从少量培养物中更高地回收细胞，且细胞细节更好。
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