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[3H-胸腺嘧啶核苷掺入原代培养的人主动脉细胞。一项放射自显影研究]

[3H-thymidine incorporation into human aorta cells in primary culture. An autoradiographic study].

作者信息

Andreeva E R, Orekhov A N, Repin V S

出版信息

Tsitologiia. 1983 Jul;25(7):849-51.

PMID:6623641
Abstract

Primary cell culture has been obtained from intima and media of unaffected zones of human aorta and from atherosclerotic lesions (fatty infiltration, fatty streaks, atherosclerotic plaques). Cellular polymorphism was found in these cultures. Four morphological types of aortic cells are described: elongated, asymmetric, polygonal, and stellate cells. These cell types also differed in their proliferative activity. On the 7th day of culturing, polygonal cells do not incorporate 3H-thymidine; the thymidine index of other three cell types was similar. The thymidine index of medial cells was higher than that of intimal ones.

摘要

原代细胞培养取自人类主动脉未受影响区域的内膜和中膜以及动脉粥样硬化病变(脂肪浸润、脂纹、动脉粥样硬化斑块)。在这些培养物中发现了细胞多态性。描述了四种主动脉细胞的形态类型:细长细胞、不对称细胞、多角形细胞和星状细胞。这些细胞类型在增殖活性方面也有所不同。在培养的第7天,多角形细胞不掺入3H-胸腺嘧啶核苷;其他三种细胞类型的胸腺嘧啶核苷指数相似。中膜细胞的胸腺嘧啶核苷指数高于内膜细胞。

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