Hamilton B T, Snyder J A
Exp Cell Res. 1983 Apr 1;144(2):345-51. doi: 10.1016/0014-4827(83)90413-5.
Mitotic PtK1 cells were treated at furrow initiation with microtubule poisons to determine the role of microtubules in the regulation of terminal cytokinetic events. The administration of anti-microtubule agents in late anaphase accelerated the rate of cytokinesis by approx. 60% as measured by changes in furrow diameter. Application of colcemid, nocodazole, or vinblastine sulfate to cells at furrow initiation all increased the rate of furrowing. Nocodazole, applied at various concentrations, demonstrated a dose-dependent relationship with furrowing rate. These results suggest a coupling between the disorganization and depolymerization of microtubules and the acceleration of furrowing. Electron microscopic analysis of cells treated with microtubule inhibitors show approx. 70% fewer microtubule profiles in the constricted region between the daughter cells. Microtubules may play a restraining role in the rate of furrowing.
用微管毒物处理处于沟形成期的有丝分裂PtK1细胞，以确定微管在终末胞质分裂事件调控中的作用。在后期晚期给予抗微管药物，以沟直径变化衡量，胞质分裂速率加快约60%。在沟形成期向细胞施加秋水仙酰胺、诺考达唑或硫酸长春碱均增加了沟形成速率。以不同浓度施加诺考达唑，显示出与沟形成速率的剂量依赖性关系。这些结果表明微管的紊乱和解聚与沟形成加速之间存在耦合。对用微管抑制剂处理的细胞进行电子显微镜分析显示，子细胞之间收缩区域的微管轮廓减少约70%。微管可能在沟形成速率中起抑制作用。
