Neff A W, Malacinski G M, Wakahara M, Jurand A
Dev Biol. 1983 May;97(1):103-12. doi: 10.1016/0012-1606(83)90068-4.
Fertile Xenopus laevis eggs were immobilized so that they were prevented from undergoing the "rotation response" to activation. Many of those unrotated eggs developed through organogenesis, indicating that egg rotation is not a prerequisite for normal early embryogenesis. Various aspects of the regulation of pattern formation were analyzed in unrotated eggs: It was discovered that a substantial rearrangement of yolk platelets occurred without affecting subsequent pattern formation. The germ plasm, however, remained localized in the vegetal hemisphere in inverted eggs. Cleavage furrows and the site of involution were both often observed in novel locations in inverted eggs which were prevented from rotating during activation.
将非洲爪蟾的可育卵固定，使其无法对激活产生“旋转反应”。许多未旋转的卵通过器官发生发育，这表明卵的旋转不是正常早期胚胎发育的先决条件。在未旋转的卵中分析了模式形成调控的各个方面：发现卵黄小板发生了大量重排，但不影响随后的模式形成。然而，生殖质在倒置卵中仍位于植物半球。在激活过程中被阻止旋转的倒置卵中，经常在新的位置观察到卵裂沟和内卷部位。
