Magnusson C, Bar Ami S, Braw R, Tsafriri A
J Reprod Fertil. 1983 May;68(1):97-103. doi: 10.1530/jrf.0.0680097.
Oxygen consumption was measured in denuded oocytes and oocyte-cumulus complexes isolated from atretic rat follicles. Adult cyclic rats or immature PMSG-treated rats were used, and follicular atresia was induced by hypophysectomy on the morning of pro-oestrus or by repeated pentobarbitone injections beginning on the day of pro-oestrus. The later stages of atresia were accompanied by meiosis-like changes in the oocytes. Oxygen consumption by oocytes that had resumed meiosis (germinal vesicle breakdown, GVB) was higher than in oocytes with an intact germinal vesicle, a change similar to that observed in oocytes maturing in healthy follicles. This may indicate that the meiotic process in the atretic follicles is similar to that in normal ones. Oxygen consumption by the cumulus cells was not altered during pentobarbitone-induced atresia. Hypophysectomy led to a rapid and marked increase in cumulus oxygen consumption in cyclic rats but there was no change in PMSG-treated young animals. Since both pentobarbitone-treatment and hypophysectomy result in follicular atresia, but changes in cumulus respiration occurred only in hypophysectomized adult rats, it is concluded that an increase in cumulus respiration is not inherent to the atretic process.
在从闭锁大鼠卵泡中分离出的裸卵母细胞和卵母细胞-卵丘复合体中测量了氧消耗。使用成年周期性大鼠或未成熟的经孕马血清促性腺激素（PMSG）处理的大鼠，在动情前期早晨通过垂体切除或从动情前期当天开始反复注射戊巴比妥来诱导卵泡闭锁。闭锁的后期伴随着卵母细胞中类似减数分裂的变化。恢复减数分裂（生发泡破裂，GVB）的卵母细胞的氧消耗高于具有完整生发泡的卵母细胞，这一变化与在健康卵泡中成熟的卵母细胞中观察到的相似。这可能表明闭锁卵泡中的减数分裂过程与正常卵泡中的相似。在戊巴比妥诱导的闭锁过程中，卵丘细胞的氧消耗没有改变。垂体切除导致周期性大鼠卵丘氧消耗迅速且显著增加，但经PMSG处理的幼龄动物没有变化。由于戊巴比妥处理和垂体切除都会导致卵泡闭锁，但卵丘呼吸的变化仅发生在垂体切除的成年大鼠中，因此得出结论，卵丘呼吸增加不是闭锁过程所固有的。
