Hirsen D J, Dubin B A, Malham L M
Int Arch Allergy Appl Immunol. 1983;71(3):241-4. doi: 10.1159/000233397.
Daily variation in natural killer (NK) and antibody-dependent cell-mediated cytotoxicity (ADCC) assays is problematic. When fresh peripheral blood mononuclear cells (PBM) from 4 donors were tested repeatedly, the ADCC and NK values tended to fall in the same rank order on each day tested, suggesting that repetitive assay of control donors could serve as an index for daily variation. Cryopreservation of PBM resulted in decreased and quite variable recovery of ADCC and NK. Cryopreservation, therefore, would not be an adequate control for daily variation. Storage of PBM at 4 degrees C for 24 h resulted in better recovery of ADCC and NK activities than did cryopreservation.
自然杀伤（NK）和抗体依赖性细胞介导的细胞毒性（ADCC）检测中的每日变化是个问题。当对来自4名供体的新鲜外周血单核细胞（PBM）进行重复检测时，在每次检测日，ADCC和NK值往往呈相同的排序下降，这表明对对照供体进行重复检测可作为每日变化的一个指标。PBM的冷冻保存导致ADCC和NK的回收率降低且变化很大。因此，冷冻保存不能作为每日变化的充分对照。将PBM在4℃保存24小时，其ADCC和NK活性的回收率比冷冻保存要好。
