Grill H J, Manz B, Belovsky O, Krawielitzki B, Pollow K
J Clin Chem Clin Biochem. 1983 Mar;21(3):175-9. doi: 10.1515/cclm.1983.21.3.175.
The newly available ligand [16 alpha-125I]3,17 beta-oestradiol was investigated for its suitability for routine oestrogen receptor determinations. Unlabeled 16 alpha-iodo-oestradiol was synthesized. No difference in the relative binding affinity to human uterine oestrogen receptor could be demonstrated, but unlabeled as well as labeled 16 alpha-iodo-oestradiol do not bind to serum sex hormone binding globulin. At 0--4 degrees C [3H]oestradiol reaches, when incubated with oestrogen receptor its equilibrium after 3 h, [125I]oestradiol after 9 h. In 100 human mammary tumour cytosols oestrogen receptor concentrations were determined using either [3H]oestradiol or [125I]oestradiol. The mean Kd-values obtained were 0.5 +/- 0.3 nmol/l using [3H]oestradiol and 0.42 +/- 0.25 nmol/l for [125I]oestradiol. For the linear regression (independent variable [3H]oestradiol, dependent variable [125I]oestradiol) the following sample estimates were obtained: n = 100, r = 0.982, yi = 3.04 + 1.00035 x. For a 0.95 probability of confidence it can be stated that the sample estimates are indeed comparable according to the criteria of the two ligands.
对新可得的配体[16α-125I]3,17β-雌二醇进行了常规雌激素受体测定适用性的研究。合成了未标记的16α-碘雌二醇。未证明其与人子宫雌激素受体的相对结合亲和力有差异，但未标记的以及标记的16α-碘雌二醇均不与血清性激素结合球蛋白结合。在0 - 4℃下，[3H]雌二醇与雌激素受体孵育3小时后达到平衡，[125I]雌二醇则在9小时后达到平衡。在100例人乳腺肿瘤胞质溶胶中，使用[3H]雌二醇或[125I]雌二醇测定雌激素受体浓度。使用[3H]雌二醇获得的平均解离常数（Kd）值为0.5±0.3nmol/L，使用[125I]雌二醇为0.42±0.25nmol/L。对于线性回归（自变量为[3H]雌二醇，因变量为[125I]雌二醇），得到以下样本估计值：n = 100，r = 0.982，yi = 3.04 + 1.00035x。对于0.95的置信概率，可以说根据两种配体的标准，样本估计值确实具有可比性。
