早期发育的蝾螈卵中8S DNA连接酶信使核糖核酸的分离及其无细胞翻译

Isolation of the messenger RNA for 8S DNA ligase in early developing axolotl egg and its cell free translation.

作者信息

Thiebaud P, Lefresne J, Signoret J, David J C

出版信息

Nucleic Acids Res. 1983 May 11;11(9):2563-73. doi: 10.1093/nar/11.9.2563.

DOI:10.1093/nar/11.9.2563

PMID:6682971

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325908/

Abstract

A new DNA ligase activity is expressed when the Axolotl eggs enter cleavage. The messenger RNA can be labelled by [3H] uridine thereby indicating its de novo synthesis. This new genetic expression is occurring just before cleavage and is the earliest found during Amphibian development. The newly synthesized [3H] mRNA can be translated in vitro in the rabbit reticulocyte lysate system. The resulting product is a 160 K protein specifically immunoprecipitated with the antiserum directed against 8S DNA ligase. This in vitro translated polypeptide exhibits 8S DNA ligase activity specific of activated or fertilized eggs but does not display 6S DNA ligase activity of non activated eggs.

摘要

当美西螈卵进入卵裂期时，会表达一种新的DNA连接酶活性。信使核糖核酸（mRNA）可用[3H]尿苷进行标记，从而表明其从头合成。这种新的基因表达恰好在卵裂之前发生，是在两栖动物发育过程中最早发现的。新合成的[3H] mRNA可在兔网织红细胞裂解物系统中进行体外翻译。产生的产物是一种160K的蛋白质，它能被针对8S DNA连接酶的抗血清特异性免疫沉淀。这种体外翻译的多肽表现出活化或受精卵特有的8S DNA连接酶活性，但不表现未活化卵的6S DNA连接酶活性。