Devaki Nandan S, Sanjeeva Rao M
Food Chem Toxicol. 1983 Jun;21(3):335-7. doi: 10.1016/0278-6915(83)90070-4.
Phenobarbital, a potent antiepileptic drug, was tested for the induction of dominant lethal mutations in male Swiss mice. The drug was administered in sterile distilled water at doses of 42, 84 or 126 mg/kg body weight by intubation over five consecutive days. Controls were given distilled water alone. The males were then mated for 1 wk sequentially with pairs of untreated virgin females for a total of 8 wk. The females were killed 17 days after presumed mating and the uterine contents were examined. There was a significant reduction in the total number of implantations and an increase in the number of dead implantations per pregnant female in the treated groups compared with the controls. The results from sequential matings showed that the pre-meiotic stages of spermatogenesis were more affected than the post-meiotic stages.
苯巴比妥是一种强效抗癫痫药物，对雄性瑞士小鼠进行了显性致死突变诱导试验。该药物以42、84或126毫克/千克体重的剂量溶于无菌蒸馏水中，通过插管连续五天给药。对照组仅给予蒸馏水。然后，将雄性小鼠与未处理的处女雌性交配1周，共持续8周。假定交配17天后处死雌性小鼠，并检查子宫内容物。与对照组相比，处理组每只怀孕雌性的着床总数显著减少，死胎着床数增加。连续交配的结果表明，精子发生的减数分裂前期阶段比减数分裂后期阶段受影响更大。
