Burov Iu V, Iukhananov R Iu, Maĭskiĭ A I
Biull Eksp Biol Med. 1983 Jul;96(7):48-51.
Radioimmunoassay was used to measure the content of leu- and met-enkephalins in white random-bred rats divided into groups according to the duration of ethanol anesthesia and the levels of 15% ethanol consumption under the conditions of free choice. The concentration of neuropeptides was determined in the cortex of the large hemispheres, striatum, thalamus, and medulla oblongata. The short-sleeping animals manifested elevated concentration of leu-enkephalin in the cortex and that of met-enkephalin in the striatum, medulla oblongata, and thalamus. Prolonged alcoholization under the conditions of free choice led, in the much-drinking animals, to decreased concentration of leu- and met-enkephalins in the striatum, thalamus and medulla oblongata and to increased concentration of leu-enkephalin in the cortex. The importance of leu- and met-enkephalins in the pathogenesis of chronic experimental alcoholism in rats with different alcoholic motivation is considered.
采用放射免疫分析法，对白色随机繁殖大鼠进行分组，根据乙醇麻醉持续时间以及在自由选择条件下15%乙醇消耗量的水平，测定亮氨酸脑啡肽和甲硫氨酸脑啡肽的含量。在大脑半球皮质、纹状体、丘脑和延髓中测定神经肽的浓度。短睡眠动物大脑皮质中亮氨酸脑啡肽浓度升高，纹状体、延髓和丘脑中甲硫氨酸脑啡肽浓度升高。在自由选择条件下长期饮酒，在大量饮酒的动物中，纹状体、丘脑和延髓中亮氨酸脑啡肽和甲硫氨酸脑啡肽浓度降低，而大脑皮质中亮氨酸脑啡肽浓度升高。研究了亮氨酸脑啡肽和甲硫氨酸脑啡肽在不同酒精成瘾动机大鼠慢性实验性酒精中毒发病机制中的重要性。
