Moss D J
Eur J Biochem. 1983 Sep 15;135(2):291-7. doi: 10.1111/j.1432-1033.1983.tb07651.x.
A non-ionic detergent-insoluble fraction was obtained from pure cultures of chicken sympathetic neurons and further purified at the 10%-30% interface of a discontinuous density gradient. This fraction contains actin as its major component and approximately 20 further polypeptides some of which are glycosylated. Two conspicuous glycoproteins in this fraction, of molecular masses 130 kDa and 90 kDa, have been shown to bind to concanavalin A; in cultured neurons the 130-kDa glycoprotein may also be labelled with [3H]glucosamine and [3H]fucose. Both are restricted to one interface of the stepped sucrose gradient when cells are lysed in low ionic strength buffer and eluted with actin in the void volume of a Sepharose 6B column. Glycoproteins of the same molecular weight have been obtained by the same isolation procedure from 10-day-old chicken embryo brains. One-dimensional peptide maps show that the carbohydrate-containing peptides from brain and sympathetic neurons are closely similar if not identical. The glycoproteins are also present in sciatic nerve but cannot be detected in a detergent-insoluble form in rounded neurons - lacking axons - or fibroblasts. They might, therefore, be involved in the linkage of the axonal cytoskeleton to the plasma membrane.
从鸡交感神经元的纯培养物中获得了一种非离子去污剂不溶性组分，并在不连续密度梯度的10%-30%界面处进一步纯化。该组分以肌动蛋白为主要成分，还含有约20种其他多肽，其中一些是糖基化的。该组分中有两种明显的糖蛋白，分子量分别为130 kDa和90 kDa，已证明它们能与伴刀豆球蛋白A结合；在培养的神经元中，130 kDa的糖蛋白也可用[3H]葡萄糖胺和[3H]岩藻糖标记。当细胞在低离子强度缓冲液中裂解并用肌动蛋白在琼脂糖6B柱的空体积中洗脱时，这两种糖蛋白都局限于阶梯状蔗糖梯度的一个界面。通过相同的分离程序，从10日龄鸡胚脑中也获得了相同分子量的糖蛋白。一维肽图显示，来自脑和交感神经元的含碳水化合物的肽即使不完全相同也非常相似。坐骨神经中也存在这些糖蛋白，但在没有轴突的圆形神经元或成纤维细胞中，无法检测到去污剂不溶性形式的糖蛋白。因此，它们可能参与轴突细胞骨架与质膜的连接。
