Giometti C S, Danon M J, Anderson N G
Neurology. 1983 Sep;33(9):1152-6. doi: 10.1212/wnl.33.9.1152.
Proteins from single frozen sections of human muscle were separated by two-dimensional gel electrophoresis and detected by fluorography or Coomassie Blue staining. The major proteins were identical in different normal muscles obtained from either sex at different ages, and in Duchenne and myotonic dystrophy samples. Congenital myopathy, denervation atrophy, polymyositis, and Becker's muscular dystrophy samples, however, showed abnormal myosin light chain compositions, some with a decrease of fast-fiber myosin light chains and others with a decrease of slow-fiber light chains. These protein alterations did not correlate with any specific disease, and may be caused by generalized muscle-fiber damage.
通过二维凝胶电泳分离人类肌肉单冻切片中的蛋白质，并通过荧光自显影或考马斯亮蓝染色进行检测。在不同年龄、不同性别的正常肌肉以及杜兴氏肌营养不良症和强直性肌营养不良症样本中，主要蛋白质是相同的。然而，先天性肌病、去神经萎缩、多发性肌炎和贝克氏肌营养不良症样本显示出肌球蛋白轻链组成异常，一些样本中快肌纤维肌球蛋白轻链减少，另一些样本中慢肌纤维轻链减少。这些蛋白质改变与任何特定疾病均无关联，可能是由广泛性肌纤维损伤引起的。
