Tissue distribution of coumarin, 7-hydroxycoumarin and their 7-hydroxy metabolites following parenteral administration of 14C-labeled compound in the DBA/lac mouse.

The time related tissue distributions of coumarin (C), 7-hydroxycoumarin (7HC) and their metabolites were studied in DBA/lac mice following retro-orbital injection of 14C-labeled compound. The activity was determined as dpm/ml or g wet weight over a period of 120 min. C was found in blood, kidney, liver, muscle, brain, heart, lung and fat but not in the testes. Upon C dosing, 7HC was found in kidney, liver and lung; 7-hydroxycoumarin glucuronide (7HCG) was found in blood, kidney and liver; 7-hydroxycoumarin sulfate (7HCS) was found in the blood, kidney and lung. Unidentified glucuronide and sulfate metabolites were detected in blood, kidney, liver and lung. Two unidentified unconjugated metabolites were detected in the kidney and liver. Unidentified water soluble metabolites were detected in kidney, liver, muscle, heart and testes. Upon 7HC dosing only 7HC, 7HCG and 7HCS were detected in any organ. 7HC and 7HCG were found in blood, kidney, liver, muscle and lung. 7HCS was detected in blood, kidney, liver and lung. Upon both C and 7HC dosing the kidney and liver contained the highest levels of radioactivity.