Munn A R, Sar M, Stumpf W E
Brain Res. 1983 Sep 5;274(1):1-10. doi: 10.1016/0006-8993(83)90515-2.
The topographic distribution of progestin concentrating cells in the female hamster brain and pituitary was studied by thaw-mount autoradiography. Fifteen minutes after injection of [3H]R5020, a synthetic progestin, nuclear uptake and concentration of radioactivity was found in certain cells of the forebrain and midbrain, as well as in the anterior pituitary. Competition studies with unlabeled R5020 abolished and with progesterone reduced the nuclear uptake of radioactivity. In the forebrain, radioactively labeled cells are observed in n. septi lateralis, n. interstitialis striae terminalis, n. preopticus medialis and lateralis, n. periventricularis hypothalami, organum subfornicale, n. arcuatus hypothalami, n. ventromedialis hypothalami, n. dorsomedialis hypothalami and n. premammillaris ventralis. In the midbrain a few labeled cells are found in the griseum centrale. In the anterior pituitary, labeled cells are identified by immunostaining as luteinizing hormone-producing cells. The results of the autoradiographic study demonstrate nuclear progestin binding sites and suggest genomic action of progestin on the labeled structures, probably related to the modulation of gonadotropin secretion and sexual behavior in the brain and pituitary.
通过冰冻切片放射自显影术研究了雌性仓鼠脑和垂体中孕激素浓缩细胞的地形分布。注射合成孕激素[3H]R5020 15分钟后，在前脑和中脑的某些细胞以及垂体前叶中发现了放射性的核摄取和浓缩。用未标记的R5020进行的竞争研究消除了放射性的核摄取，而用孕酮进行的竞争研究则降低了放射性的核摄取。在前脑中，在外侧隔核、终纹间质核、内侧和外侧视前核、下丘脑室周核、穹窿下器官、下丘脑弓状核、下丘脑腹内侧核、下丘脑背内侧核和腹侧乳头前核中观察到放射性标记细胞。在中脑的中央灰质中发现了一些标记细胞。在垂体前叶中，通过免疫染色将标记细胞鉴定为产生促黄体生成素的细胞。放射自显影研究结果表明存在核孕激素结合位点，并提示孕激素对标记结构有基因组作用，这可能与调节脑和垂体中的促性腺激素分泌及性行为有关。
