一种获取人类精子染色体标本的详细方法。
A detailed method for obtaining preparations of human sperm chromosomes.
作者信息
Martin R H
出版信息
Cytogenet Cell Genet. 1983;35(4):252-6. doi: 10.1159/000131876.
PMID:6684539
Abstract
A detailed technique is described for obtaining preparations of the chromosome complements of human sperm by fertilization of hamster eggs and analysis of the male pronucleus. Some of the more difficult aspects and important steps are emphasized. Technical data from 17 consecutive experiments are presented to provide an estimate of the number of karyotypes which can be obtained in an experiment.
摘要
本文描述了一种通过仓鼠卵受精和雄性原核分析来获取人类精子染色体组制备物的详细技术。强调了一些较为困难的方面和重要步骤。给出了连续17次实验的技术数据，以估计一次实验中可获得的核型数量。
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