6-酮前列地尔1的分解代谢：大鼠肾脏在体外的生物激活作用

Catabolism of 6 keto PGE1: biological activation by the rat kidney in vitro.

作者信息

Moore P K, Griffiths R J, Gaffen Z A

出版信息

Eur J Pharmacol. 1983 Jul 22;91(2-3):311-5. doi: 10.1016/0014-2999(83)90485-5.

Abstract

An increase in anti-aggregatory but not spasmogenic activity was observed when 6 keto prostaglandin E1 (but not PGE1) was incubated at 37 degrees C with rat kidney 100 000 X g supernatant. No such biological activation was observed in boiled rat kidney supernatant. After high-pressure liquid chromatography two absorbance peaks with anti-aggregatory activity were detected. One peak had a retention time identical to authentic 6 keto prostaglandin E1 whilst the second peak did not coincide with known anti-aggregatory prostaglandins.

摘要

当6-酮基前列腺素E1（而非前列腺素E1）与大鼠肾脏100 000×g上清液在37℃孵育时，观察到抗聚集活性增加，但痉挛原活性未增加。在煮沸的大鼠肾脏上清液中未观察到这种生物活化。高压液相色谱后检测到两个具有抗聚集活性的吸光度峰。一个峰的保留时间与 authentic 6-酮基前列腺素E1相同，而第二个峰与已知的抗聚集前列腺素不一致。