Yamakado T, Nishikawa M, Azuma H, Hidaka H
Eur J Pharmacol. 1983 Aug 5;91(4):441-7. doi: 10.1016/0014-2999(83)90168-1.
The pharmacological properties of human platelet strips were studied by recording changes in tension after the application of various compounds. The platelet strips were prepared by packing cold re-calcified human platelets over a sheer gelatinized nylon mesh. The contractile response of such a strip was dependent on the number of platelets sticking to the nylon mesh. The strip was contracted by the addition of platelet aggregation inducers such as ADP, norepinephrine, thrombin and collagen, thereby suggesting that the contractile response is mediated through membrane receptors on the platelets. The ED50 values of the aggregation agents which contracted the strip were much the same as those which induced platelet aggregation, thereby suggesting that contraction of the platelet strip is similar to platelet aggregation in platelet rich plasma (PRP). The addition of platelet aggregation inhibitors such as papaverine, W-7 and PGE1 relaxed the platelet strip. The ED50 values of the aggregation inhibitors which relaxed the platelet strip were also similar to those for platelet aggregation inhibition. The time course of the release of the preloaded [14C]serotonin from the platelet strips correlated well with that of the tension developed.
通过记录施加各种化合物后张力的变化，研究了人血小板条的药理特性。血小板条是通过将冷的重新钙化的人血小板堆积在透明的明胶化尼龙网上制备的。这种条带的收缩反应取决于粘附在尼龙网上的血小板数量。通过添加血小板聚集诱导剂如ADP、去甲肾上腺素、凝血酶和胶原蛋白，条带会收缩，从而表明收缩反应是通过血小板上的膜受体介导的。使条带收缩的聚集剂的ED50值与诱导血小板聚集的聚集剂的ED50值大致相同，从而表明血小板条带的收缩类似于富含血小板血浆（PRP）中的血小板聚集。添加血小板聚集抑制剂如罂粟碱、W-7和PGE1可使血小板条带松弛。使血小板条带松弛的聚集抑制剂的ED50值也与血小板聚集抑制的ED50值相似。预加载的[14C]血清素从血小板条带中释放的时间进程与所产生的张力的时间进程密切相关。
