Southern L L, Baker D H
J Anim Sci. 1983 Aug;57(2):402-12. doi: 10.2527/jas1983.572402x.
Four experiments were conducted with an arginine (Arg)-deficient, semipurified diet to determine the Arg requirement of the weanling pig. The semipurified diet contained .18% bioavailable Arg as determined by a chick bioassay procedure and, when properly supplemented with Arg, allowed rat growth equal to that obtained with a 19.2% protein corn-soybean meal diet. A preliminary experiment with pigs indicated that the semi-purified diet was markedly deficient in Arg. The Arg requirement of the pig was estimated by adding L-Arg to the semipurified diet such that final dietary Arg levels were .18, .23, .28, .38 and .48% (Exp. 2 and 3) or .48, .58 and .68% (Exp. 4). Weight gain and efficiency of feed utilization were maximized at .48% dietary Arg. Plasma urea-N was increased and plasma NH3-N decreased by .48% Arg compared with lower levels. A slight orotic aciduria was observed in pigs fed Arg levels below .48%. From these results, it is concluded that the Arg requirement of the weanling pig is .48% of the diet, a level twice the current NRC estimate.
进行了四项实验,采用缺乏精氨酸(Arg)的半纯化日粮来确定断奶仔猪的精氨酸需求量。通过雏鸡生物测定法确定,该半纯化日粮含有0.18%的可利用精氨酸,当适当补充精氨酸时,其能使大鼠的生长速度与含19.2%蛋白质的玉米 - 豆粕日粮所达到的生长速度相同。一项针对仔猪的初步实验表明,该半纯化日粮明显缺乏精氨酸。通过向半纯化日粮中添加L - 精氨酸来估计仔猪的精氨酸需求量,使最终日粮中的精氨酸水平分别为0.18%、0.23%、0.28%、0.38%和0.48%(实验2和3),或0.48%、0.58%和0.68%(实验4)。日粮精氨酸水平为0.48%时,体重增加和饲料利用效率达到最大值。与较低水平相比,日粮中添加0.48%精氨酸会使血浆尿素氮增加,血浆氨氮降低。在饲喂精氨酸水平低于0.48%的仔猪中观察到轻微的乳清酸尿症。根据这些结果得出结论,断奶仔猪的精氨酸需求量为日粮的0.48%,这一水平是当前美国国家研究委员会(NRC)估计值的两倍。