Kaiho T, Sekiya S, Shirotake S, Takamizawa H
Nihon Sanka Fujinka Gakkai Zasshi. 1983 Sep;35(9):1611-6.
The sensitivity to methotrexate (MTX) and mechanisms of the drug resistance of seven kinds of human choriocarcinoma cell lines were studied in vitro. The results were as follows. Each cell line of HCCM-5 , BeWo, IMa, and NUC-1 secreted more than 200ng/10(6) cells/48 hours of human chorionic gonadotropin (hCG), whereas GCH-1, ENAMI-1, and SCH secreted less than 20ng/10(6) cells/48 hours. HCCM-5 cell line was the most sensitive to MTX judging from the inhibition of both 3H-thymidine uptake and cell growth in vitro. Each cell line of BeWo, IMa, and NUC-1 revealed sensitivity to 2 X 10(-8)M MTX, but such cell lines as GCH-1, ENAMI-1, and SCH showed resistance to MTX. The cell lines which revealed sensitivity to MTX incorporated more 3H-MTX and showed less intracellular dihydrofolate reductase activity than resistant cell lines.
体外研究了7种人绒毛膜癌细胞系对甲氨蝶呤（MTX）的敏感性及耐药机制。结果如下。HCCM - 5、BeWo、IMa和NUC - 1每种细胞系每48小时分泌超过200ng/10⁶细胞的人绒毛膜促性腺激素（hCG），而GCH - 1、ENAMI - 1和SCH每48小时分泌少于20ng/10⁶细胞。从体外对³H - 胸腺嘧啶核苷摄取和细胞生长的抑制作用判断，HCCM - 5细胞系对MTX最敏感。BeWo、IMa和NUC - 1每种细胞系对2×10⁻⁸M MTX敏感，但GCH - 1、ENAMI - 1和SCH等细胞系对MTX耐药。对MTX敏感的细胞系比耐药细胞系摄取更多的³H - MTX，且细胞内二氢叶酸还原酶活性更低。
