人类红细胞核苷转运体和葡萄糖转运体均为4.5带膜多肽。
The human erythrocyte nucleoside and glucose transporters are both band 4.5 membrane polypeptides.
作者信息
Wu J S, Jarvis S M, Young J D
出版信息
Biochem J. 1983 Sep 15;214(3):995-7. doi: 10.1042/bj2140995.
Abstract
Human erythrocyte membranes and partially purified nucleoside transporter (band 4.5 and 7) were photoaffinity labelled with 3H-labelled 6-[(4-nitrobenzyl)thio]-9-beta-D-ribofuranosylpurine under equilibrium binding conditions. Band 4.5 was the major site of radiolabelling in both preparations. These experiments provide additional evidence to implicate band 4.5 polypeptides in nucleoside permeation, proteins previously shown to be involved in hexose transport.
摘要
在平衡结合条件下，用人红细胞膜和部分纯化的核苷转运体（4.5带和7带）与3H标记的6-[(4-硝基苄基)硫基]-9-β-D-呋喃核糖基嘌呤进行光亲和标记。在两种制剂中，4.5带都是放射性标记的主要位点。这些实验提供了额外的证据，表明4.5带多肽参与核苷通透，此前已证明这些蛋白质参与己糖转运。
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本文引用的文献
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Nucleoside transport in human erythrocytes. Apparent molecular weight of the nitrobenzylthioinosine-binding complex estimated by radiation-inactivation analysis.人红细胞中的核苷转运。通过辐射失活分析估算的硝基苄硫代肌苷结合复合物的表观分子量。
Biochem J. 1980 Aug 15;190(2):373-6. doi: 10.1042/bj1900373.
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Extraction and partial purification of the nucleoside-transport system from human erythrocytes based on the assay of nitrobenzylthioinosine-binding activity.基于对硝基苄硫代肌苷结合活性的测定，从人红细胞中提取和部分纯化核苷转运系统。
Biochem J. 1981 Jan 15;194(1):331-9. doi: 10.1042/bj1940331.
3
Cytochalasin B. A natural photoaffinity ligand for labeling the human erythrocyte glucose transporter.细胞松弛素B。一种用于标记人类红细胞葡萄糖转运蛋白的天然光亲和配体。
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4
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J Biol Chem. 1982 May 25;257(10):5419-25.
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Nucleoside transport in animal cells.动物细胞中的核苷转运
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Reconstitution and purification of the D-glucose transporter from human erythrocytes.人红细胞D-葡萄糖转运蛋白的重组与纯化。
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