人类红细胞核苷转运体和葡萄糖转运体均为4.5带膜多肽。
The human erythrocyte nucleoside and glucose transporters are both band 4.5 membrane polypeptides.
作者信息
Wu J S, Jarvis S M, Young J D
出版信息
Biochem J. 1983 Sep 15;214(3):995-7. doi: 10.1042/bj2140995.
Abstract
Human erythrocyte membranes and partially purified nucleoside transporter (band 4.5 and 7) were photoaffinity labelled with 3H-labelled 6-[(4-nitrobenzyl)thio]-9-beta-D-ribofuranosylpurine under equilibrium binding conditions. Band 4.5 was the major site of radiolabelling in both preparations. These experiments provide additional evidence to implicate band 4.5 polypeptides in nucleoside permeation, proteins previously shown to be involved in hexose transport.
摘要
在平衡结合条件下，用人红细胞膜和部分纯化的核苷转运体（4.5带和7带）与3H标记的6-[(4-硝基苄基)硫基]-9-β-D-呋喃核糖基嘌呤进行光亲和标记。在两种制剂中，4.5带都是放射性标记的主要位点。这些实验提供了额外的证据，表明4.5带多肽参与核苷通透，此前已证明这些蛋白质参与己糖转运。
相似文献
1
The human erythrocyte nucleoside and glucose transporters are both band 4.5 membrane polypeptides.
Biochem J. 1983 Sep 15;214(3):995-7. doi: 10.1042/bj2140995.
2
Identification of glucose and nucleoside transport proteins in neonatal pig erythrocytes using monoclonal antibodies against band 4.5 polypeptides of adult human and pig erythrocytes.
Biochem Cell Biol. 1988 Aug;66(8):839-52. doi: 10.1139/o88-096.
3
Proteolytic cleavage of [3H]nitrobenzylthioinosine-labelled nucleoside transporter in human erythrocytes.
Biochem J. 1985 Sep 15;230(3):777-84. doi: 10.1042/bj2300777.
4
Chromatographic characterization of nitrobenzylthioinosine binding proteins in band 4.5 of human erythrocytes: purification of a 40 kDa truncated nucleoside transporter.
Biochim Biophys Acta. 1990 Oct 19;1028(3):251-60. doi: 10.1016/0005-2736(90)90174-m.
5
Identification of the erythrocyte nucleoside transporter as a band 4.5 polypeptide. Photoaffinity labeling studies using nitrobenzylthioinosine.
J Biol Chem. 1983 Nov 25;258(22):13745-51.
6
5'-S-(2-aminoethyl)-N6-(4-nitrobenzyl)-5'-thioadenosine (SAENTA), a novel ligand with high affinity for polypeptides associated with nucleoside transport. Partial purification of the nitrobenzylthioinosine-binding protein of pig erythrocytes by affinity chromatography.
Biochem J. 1990 Sep 15;270(3):605-14. doi: 10.1042/bj2700605.
7
Kinetics of nitrobenzylthioinosine binding to the human erythrocyte nucleoside transporter.
Biochem J. 1983 Dec 15;216(3):661-7. doi: 10.1042/bj2160661.
8
Extraction and partial purification of the nucleoside-transport system from human erythrocytes based on the assay of nitrobenzylthioinosine-binding activity.
Biochem J. 1981 Jan 15;194(1):331-9. doi: 10.1042/bj1940331.
9
Characterization of monoclonal antibodies that recognize band 4.5 polypeptides associated with nucleoside transport in pig erythrocytes.
Biochem J. 1987 Jun 15;244(3):749-55. doi: 10.1042/bj2440749.
10
Cytochalasin B. A natural photoaffinity ligand for labeling the human erythrocyte glucose transporter.
J Biol Chem. 1982 Jul 10;257(13):7290-3.
引用本文的文献
1
ADP modifies the function of the glucose transporter: studies with reconstituted liposomes.
Biochem J. 1993 Jun 15;292 ( Pt 3)(Pt 3):877-81. doi: 10.1042/bj2920877.
2
Photoaffinity labelling of nucleoside-transport proteins in plasma membranes isolated from rat and guinea-pig liver.
Biochem J. 1984 Jun 1;220(2):499-506. doi: 10.1042/bj2200499.
3
Proteolytic cleavage of [3H]nitrobenzylthioinosine-labelled nucleoside transporter in human erythrocytes.
Biochem J. 1985 Sep 15;230(3):777-84. doi: 10.1042/bj2300777.
4
Inhibition by nucleosides of glucose-transport activity in human erythrocytes.
Biochem J. 1988 Jan 15;249(2):383-9. doi: 10.1042/bj2490383.
5
Erythrocyte nucleoside and sugar transport. Endo-beta-galactosidase and endoglycosidase-F digestion of partially purified human and pig transporter proteins.
Biochem J. 1986 Dec 1;240(2):349-56. doi: 10.1042/bj2400349.
6
Nucleoside transport in rat erythrocytes: two components with differences in sensitivity to inhibition by nitrobenzylthioinosine and p-chloromercuriphenyl sulfonate.
J Membr Biol. 1986;93(1):1-10. doi: 10.1007/BF01871013.
7
Possible multifunction of glucose transporter. Transport of nicotinamide by reconstituted liposomes.
Biochem J. 1992 Dec 1;288 ( Pt 2)(Pt 2):669-74. doi: 10.1042/bj2880669.
本文引用的文献
1
Nucleoside transport in human erythrocytes. Apparent molecular weight of the nitrobenzylthioinosine-binding complex estimated by radiation-inactivation analysis.
Biochem J. 1980 Aug 15;190(2):373-6. doi: 10.1042/bj1900373.
2
Extraction and partial purification of the nucleoside-transport system from human erythrocytes based on the assay of nitrobenzylthioinosine-binding activity.
Biochem J. 1981 Jan 15;194(1):331-9. doi: 10.1042/bj1940331.
3
Cytochalasin B. A natural photoaffinity ligand for labeling the human erythrocyte glucose transporter.
J Biol Chem. 1982 Jul 10;257(13):7290-3.
4
Photoaffinity labeling of the human erythrocyte D-glucose transporter.
J Biol Chem. 1982 May 25;257(10):5419-25.
5
6
Nucleoside transport in animal cells.
Biosci Rep. 1983 Apr;3(4):309-22. doi: 10.1007/BF01122895.
7
Reconstitution and purification of the D-glucose transporter from human erythrocytes.
J Biol Chem. 1977 Oct 25;252(20):7384-90.
Zotero 插件