Schoots A F, Ruijter J M, van Kemenade J A, Denucé J M
Cell Tissue Res. 1983;233(3):611-8. doi: 10.1007/BF00212228.
In the developing pituitary gland of embryos of the annual fish Cynolebias whitei and the medaka, Oryzias latipes, prolactin cells have been identified before hatching by means of a light-microscopic immunocytochemical method with antiserum against ovine prolactin. At the time of hatching, changes in the intensity of the immunoperoxidase staining occur. Histological staining by Cleveland and Wolfe's trichrome shows differentiation of cell types in the adenohypophysis only later in ontogeny. Our results indicate that, in teleosts, differentiated prolactin cells are present before hatching and that prolactin may be involved in the endocrine control of the hatching process.
在一年生鱼类怀氏辛氏鳉和青鳉胚胎发育中的垂体中，通过使用抗绵羊催乳素的抗血清的光学显微镜免疫细胞化学方法，在孵化前已鉴定出催乳素细胞。在孵化时，免疫过氧化物酶染色强度发生变化。克利夫兰和沃尔夫三色染色法的组织学染色显示，腺垂体中的细胞类型仅在个体发育后期才出现分化。我们的结果表明，在硬骨鱼类中，分化的催乳素细胞在孵化前就已存在，并且催乳素可能参与孵化过程的内分泌控制。
