Miettinen M, Partanen S, Lehto V P, Virtanen I
Ultrastruct Pathol. 1983 Jun;4(4):337-47. doi: 10.3109/01913128309140586.
The histogenesis of six mediastinal tumors was investigated ultrastructurally and immunohistochemically using monospecific antibodies against intermediate filament proteins. Four of the tumors, showing different appearances by light microscopy, displayed desmosomes and cytoplasmic tonofilaments, by electron microscopy, compatible with an epithelial thymoma. These cases also showed keratin positivity by immunofluorescence microscopy. One spindle cell tumor showed zonula adherens-type junctions, prominent collections of intermediate filaments, and abundant cytoplasmic neurosecretory granules consistent with a neuroendocrine tumor. In this tumor, neurofilaments could be demonstrated by immunofluorescence microscopy, a feature also consistent with a neuroendocrine tumor. One malignant tumor, lacking tonofilaments and desmosomes but showing a few primitive junctions, did not contain keratin but showed vimentin positivity. This suggests a mesenchymal origin and a diagnosis of primitive sarcoma. These cases illustrate the diagnostic usefulness of electron microscopy and immunohistochemical evaluation of intermediate filaments.
利用抗中间丝蛋白的单特异性抗体，对6例纵隔肿瘤进行了超微结构和免疫组织化学研究。其中4例肿瘤在光学显微镜下表现各异，通过电子显微镜观察显示有桥粒和细胞质张力丝，符合上皮性胸腺瘤。这些病例在免疫荧光显微镜下也显示角蛋白呈阳性。1例梭形细胞瘤显示黏着小带型连接、大量中间丝聚集以及丰富的细胞质神经分泌颗粒，符合神经内分泌肿瘤。在该肿瘤中，免疫荧光显微镜可显示神经丝，这一特征也与神经内分泌肿瘤相符。1例恶性肿瘤缺乏张力丝和桥粒，但有一些原始连接，不含角蛋白但波形蛋白呈阳性。这提示其起源于间充质，诊断为原始肉瘤。这些病例说明了电子显微镜检查和中间丝免疫组织化学评估在诊断中的实用性。
