Olson E B, Rankin J
Biol Neonate. 1983;44(6):366-71. doi: 10.1159/000241752.
A 20-second bolus of serotonin (5-hydroxytryptamine, 5HT), prostaglandin E1 (PGE1) or norepinephrine (NE) was infused into the pulmonary arteries of lungs isolated from 26-day gestation fetal, newborn (within 12 h of birth), and young adult rabbits. In each case the concentration of the infused substrate was much less than the apparent Michaelis constant for the uptake process. At these low concentrations there was no observable vasoactivity. In order to assure a constant ratio of flow to capillary surface area (independent of lung size), perfusate flow was adjusted so that the retention time of a vascular dye was approximately 7 s. Under these conditions the isolated adult rabbit lungs inactivate a similar fraction (40-50%) of each of the substrates. In contrast, isolated newborn rabbit lungs inactivate about 20% of the infused PGE1, 90% of the 5HT, and 60% of the NE, and the nonventilated, previable fetal rabbit lungs inactivate about 20% of the infused PGE1, 60% of the 5HT, and 80% of the NE.
将20秒的血清素（5-羟色胺，5HT）、前列腺素E1（PGE1）或去甲肾上腺素（NE）推注到从妊娠26天的胎儿、新生（出生后12小时内）和成年幼兔分离的肺的肺动脉中。在每种情况下，注入底物的浓度远低于摄取过程的表观米氏常数。在这些低浓度下，没有观察到血管活性。为了确保流量与毛细血管表面积的恒定比例（与肺大小无关），调整灌注液流量，使血管染料的保留时间约为7秒。在这些条件下，分离的成年兔肺使每种底物的失活比例相似（40-50%）。相比之下，分离的新生兔肺使注入的PGE1失活约20%，5HT失活90%，NE失活60%，未通气的、未成熟的胎儿兔肺使注入的PGE1失活约20%，5HT失活60%，NE失活80%。
