Yoshida N, Sekiba K, Shibusawa H, Sano Y, Yanaihara T, Okinaga S, Arai K
Endocrinol Jpn. 1978 Apr;25(2):191-5. doi: 10.1507/endocrj1954.25.191.
Eleven incubations of minced human fetal adrenal tissue from two fetuses at midtrimester of pregnancy terminated for socio-economic reasons, were performed for different periods of time with labelled pregnenolone, progesterone, 17alpha-hydroxyprogesterone and deoxycorticosterone (DOC). The order of magnitudes of enzyme activity when progesterone was used as a substrate and incubated with midterm fetal adrenals was 17alpha-hydroxylase, 21-hydroxylase and 16alpha-hydroxylase. The activity of 3beta-hydroxysteroid dehydrogenase and delta5-4 isomerase was almost negligible as compared to other enzymic activities as mentioned above. Similar biosynthetic pathways for adrenal corticoids formation were observed in human fetal adrenals as reported in human adults and animals; once progesterone was converted into DOC, further 17alpha-hydroxylation did not take place.
因社会经济原因，对妊娠中期两个胎儿的人胎儿肾上腺组织碎块进行了11次培养，分别用标记的孕烯醇酮、孕酮、17α-羟孕酮和脱氧皮质酮（DOC）培养不同时间。以孕酮为底物与中期胎儿肾上腺一起培养时，酶活性的大小顺序为17α-羟化酶、21-羟化酶和16α-羟化酶。与上述其他酶活性相比，3β-羟类固醇脱氢酶和δ5-4异构酶的活性几乎可以忽略不计。在人胎儿肾上腺中观察到的肾上腺皮质激素形成的生物合成途径与人类成年人和动物中报道的相似；一旦孕酮转化为DOC，就不会进一步发生17α-羟化。
